Microalgae Culture Growth Medium

If you are looking for a source of quality culture growth medium then you've come to the right place. We manufacture our mediums and can create any custom combinations you are interested in. Our more popular culture mediums include Guillard's F/2 (including reduced nitrogen) which is used for many open and coastal water strains of microalgae including Nannochloropsis oculata and Isochrysis galbana, and Zarrouk's SM, SMM and RM6 mediums designed for Arthrospira platensis (Spirulina). Use the tabs below to read about and purchase the mediums we manufacture.

All of our mediums are manufactured using distilled/deionized water (dH2O) and highest possible grades of chemicals (A.C.S, Reagent and USP).

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Guillard's f/2 Marine Enriched Seawater is a common and most widely used culture medium designed for growing marine microalgae. The concentration of the original formulation, termed "f Medium" (Guillard & Ryther, 1962) has been reduced by half (Guillard, 1975). The original medium (Guillard & Ryther, 1962) used ferric sequestrene; we have substituted Na₂EDTA·2H2O and FeCl₃·6H2O.

WARNING:
  • If you DO NOT culture diatoms or specifically DO NOT require Silica, omit to reduce precipitation!
  • Reduced amounts of Nitrogen can significantly raise lipid content, lower biomass production or produce other unexpected results, including a culture crash. Recommended for experienced aquaculturists only!
  • Dilute using distilled/deionized water (dH2O) only!

Our f/2 Products:
  • f/2 Concentrate¹
  • f/2 Concentrate w/Silica
  • f/2 Concentrate, 75-50-25 Nitrogen²
¹ Not suitable for diatoms
² Reduced Nitrogen, number indicates percent of required Nitrogen


Directions:

For f/2 strength add 0.25 ml (5 drops) of our f/2 concentrate per liter of sterilized seawater. For "f Medium" strength add 0.5 ml (10 drops) per liter of sterilized seawater.

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Item Price Qty
f/2 Concentrate (4 oz) ~480 liters
$15.99 Spin UpSpin Down  
f/2 Concentrate (8 oz) ~960 liters
$22.99 Spin UpSpin Down  
f/2 Concentrate (16 oz) ~1920 liters
$37.99 Spin UpSpin Down  
f/2 Concentrate (32 oz) ~3840 liters
$66.99 Spin UpSpin Down  
f/2 Concentrate w/Silica (4 oz) ~480 liters
$15.99 Spin UpSpin Down  
f/2 Concentrate w/Silica (8 oz) ~960 liters
$22.99 Spin UpSpin Down  
f/2 Concentrate w/Silica (16 oz) ~1920 liters
$37.99 Spin UpSpin Down  
f/2 Concentrate w/Silica (32 oz) ~3840 liters
$66.99 Spin UpSpin Down  
f/2 Concentrate 75-Nitrogen (4 oz) ~480 liters
$15.99 Spin UpSpin Down  
f/2 Concentrate 75-Nitrogen (8 oz) ~960 liters
$22.99 Spin UpSpin Down  
f/2 Concentrate 75-Nitrogen (16 oz) ~1920 liters
$37.99 Spin UpSpin Down  
f/2 Concentrate 75-Nitrogen (32 oz) ~3840 liters
$66.99 Spin UpSpin Down  
f/2 Concentrate 50-Nitrogen (4 oz) ~480 liters
$15.99 Spin UpSpin Down  
f/2 Concentrate 50-Nitrogen (8 oz) ~960 liters
$22.99 Spin UpSpin Down  
f/2 Concentrate 50-Nitrogen (16 oz) ~1920 liters
$37.99 Spin UpSpin Down  
f/2 Concentrate 50-Nitrogen (32 oz) ~3840 liters
$66.99 Spin UpSpin Down  
f/2 Concentrate 25-Nitrogen (4 oz) ~480 liters
$15.99 Spin UpSpin Down  
f/2 Concentrate 25-Nitrogen (8 oz) ~960 liters
$22.99 Spin UpSpin Down  
f/2 Concentrate 25-Nitrogen (16 oz) ~1920 liters
$37.99 Spin UpSpin Down  
f/2 Concentrate 25-Nitrogen (32 oz) ~3840 liters
$66.99 Spin UpSpin Down  

Zarrouk's Spirulina Medium is one of the oldest and proven mediums designed for growing different microalgae strains of Arthrospira (Spirulina) genus. Using this medium you can achieve high biomass production, better β-carotene synthesis and increased antioxidant potential of all Arthrospira strains.

Item Price Qty
SM Concentrate-20 makes 20 liters of working solution
$29.99 Spin UpSpin Down  
SM Concentrate-40 makes 40 liters of working solution
$49.99 Spin UpSpin Down  
SM Concentrate-100 makes 100 liters of working solution
$99.99 Spin UpSpin Down  
SM Concentrate-4 makes 4 liters of working solution
$12.99 Spin UpSpin Down  

Schlösser Medium, Modified was modified from Spirulina platenses medium of Ogawa and Terui (1970) to prevent precipitation (Aiba and Ogawa 1977, Schlösser 1994).
 

Item Price Qty
SMM Concentrate-10 makes 10 liters of working solution
$14.99 Spin UpSpin Down  
SMM Concentrate-40 makes 40 liters of working solution
$39.99 Spin UpSpin Down  
SMM Concentrate-100 makes 100 liters of working solution
$69.99 Spin UpSpin Down  

RM6 was created as an alternative to Zarrouk's SM medium for a mass-production of Spirulina. Deprived of some mineral ingredients, it is not as beneficial to β-carotene synthesis as Zarrouk's SM but it enables a much more efficient synthesis of phycobiliproteins, which are being used by food and farmaceutical industries.

Item Price Qty
RM6 Concentrate-10 makes 10 liters of working solution
$14.99 Spin UpSpin Down  
RM6 Concentrate-40 makes 40 liters of working solution
$39.99 Spin UpSpin Down  
RM6 Concentrate-100 makes 100 liters of working solution
$69.99 Spin UpSpin Down  
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